Since the time hydrogen peroxide has been introduced in the markets, there has been a steady increase in the things this wonder chemical ...
Hydrogen peroxide agarose gels for electrophoretic analysis of RNA.
- Citation data:
Analytical biochemistry, ISSN: 1096-0309, Vol: 534, Page: 24-27
- Publication Year:
- Biochemistry, Genetics and Molecular Biology
- Most Recent Tweet View All Tweets
- Most Recent News Mention
Efficient electrophoretic separation of isolated total RNA utilizes chemicals and agents to aid in nuclease free environment. However cost, extensive pre-run processing protocols as well as toxic byproducts limit the usage of such protocols. Moreover, these treatments affect the overall electrophoretic results by altering the conductivity of the running buffer and weaken the gel strength. We here provide a protocol for RNA visualization that obviates these shortcomings by preparation of agarose gel with hydrogen peroxide using the regular TAE buffer. The simple, inexpensive protocol exhibits superior results in a horizontal agarose gel electrophoresis.