Novel mechanism of cannabidiol-induced apoptosis in breast cancer cell lines.

Citation data:

Breast (Edinburgh, Scotland), ISSN: 1532-3080, Vol: 41, Page: 34-41

Publication Year:
2018
Captures 26
Readers 26
Social Media 80
Tweets 54
Shares, Likes & Comments 26
PMID:
30007266
DOI:
10.1016/j.breast.2018.06.009
Author(s):
Sultan, Ahmed S; Marie, Mona A; Sheweita, Salah A
Publisher(s):
Elsevier BV
Tags:
Medicine
Most Recent Tweet View All Tweets
article description
Studies have emphasized an antineoplastic effect of the non-psychoactive, phyto-cannabinoid, Cannabidiol (CBD). However, the molecular mechanism underlying its antitumor activity is not fully elucidated. Herein, we have examined the effect of CBD on two different human breast cancer cell lines: the ER-positive, well differentiated, T-47D and the triple negative, poor differentiated, MDA-MB-231 cells. In both cell lines, CBD inhibited cell survival and induced apoptosis in a dose dependent manner as observed by MTT assay, morphological changes, DNA fragmentation and ELISA apoptosis assay. CBD-induced apoptosis was accompanied by down-regulation of mTOR, cyclin D1 and up-regulation and localization of PPARγ protein expression in the nuclei and cytoplasmic of the tested cells. The results suggest that CBD treatment induces an interplay among PPARγ, mTOR and cyclin D1 in favor of apoptosis induction in both ER-positive and triple negative breast cancer cells, proposing CBD as a useful treatment for different breast cancer subtypes.