Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes.

Citation data:

Genomics data, ISSN: 2213-5960, Vol: 11, Page: 113-115

Publication Year:
2017
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PMID:
28119819
DOI:
10.1016/j.gdata.2016.12.005
Author(s):
Gokoolparsadh, Akira; Fang, Zhiming; Braidy, Nady; Voineagu, Irina
Publisher(s):
Elsevier BV
Tags:
Biochemistry, Genetics and Molecular Biology
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article description
Topoisomerase I is required for the proper expression of long genes (> 100 kb) in mouse and human cortical neurons, including many candidate genes for autism spectrum disorder (ASD) [1]. Given the important role of astrocytes in brain development [2], we investigated whether long genes, including autism susceptibility genes, also require topoisomerase I expression in human primary astrocytes. We carried genome-wide expression profiling of cultured human primary astrocytes following treatment with the topoisomerase I inhibitor Topotecan, using Illumina microarrays. We identified several thousands of differentially expressed genes and confirmed that topoisomerase I inhibition affects gene expression in human primary astrocytes in a length-dependent manner. We also identified over 20 ASD-associated genes that show topoisomerase-dependent gene expression in human primary astrocytes but have not been previously reported as topoisomerase-I-dependent in neurons. The microarray data have been deposited in NCBI GEO (https://www.ncbi.nlm.nih.gov/geo/) under accession number GSE90052.