Expression of the heterologous Dunaliella tertiolecta fatty acyl-ACP thioesterase leads to increased lipid production in Chlamydomonas reinhardtii.

Citation data:

Journal of biotechnology, ISSN: 1873-4863, Vol: 247, Page: 60-67

Publication Year:
2017
Usage 29
Abstract Views 29
Social Media 98
Shares, Likes & Comments 98
Citations 2
Citation Indexes 2
PMID:
28279815
DOI:
10.1016/j.jbiotec.2017.03.004
Author(s):
Tan, Kenneth Wei Min, Lee, Yuan Kun
Publisher(s):
Elsevier BV
Tags:
Biochemistry, Genetics and Molecular Biology, Immunology and Microbiology
article description
Biofuel production from genetically-engineered microalgae is currently among the most widely studied strategies in generating renewable energy. However, microalgae currently suffer from low oil yields which limit the commercial feasibility of industrial-scale production. A major bottleneck in cost-efficient biofuel production from microalgae is the dilemma between biomass productivity and lipid accumulation. When grown under stressful culture conditions such as nitrogen depletion, microalgae accumulate large amounts of neutral lipids, but it comes at the expense of growth which negatively impacts overall lipid productivity. Overexpression of acyl-ACP thioesterases (TE) had been successful in increasing the production of fatty acids (FA) in prokaryotes such as E. coli and cyanobacteria, but has not been effectively tested in microalgae. In this study, we introduced a TE from D. tertiolecta (DtTE) into C. reinhardtii to investigate its effects on FA production without compromising growth. The results indicate that C. reinhardtii transformants were able to produce 63 and 94% more neutral lipids than the wild-type, which translates to an approximately 56% improvement in total lipids, without compromising growth. These findings demonstrate the cross-species functionality of TE, and provide a platform for further studies into using TE as a strategy to increase biofuel production from microalgae.

This article has 0 Wikipedia mention.