Genome-wide bisulfite sensitivity profiling of yeast suggests bisulfite inhibits transcription.

Citation data:

Mutation research, ISSN: 1873-135X, Vol: 821, Page: 13-19

Publication Year:
2017
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Abstract Views 93
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PMID:
28735739
DOI:
10.1016/j.mrgentox.2017.06.004
Author(s):
Segovia, Romulo, Mathew, Veena, Tam, Annie S, Stirling, Peter C
Publisher(s):
Elsevier BV
Tags:
Biochemistry, Genetics and Molecular Biology, Environmental Science
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article description
Bisulfite, in the form of sodium bisulfite or metabisulfite, is used commercially as a food preservative. Bisulfite is used in the laboratory as a single-stranded DNA mutagen in epigenomic analyses of DNA methylation. Recently it has also been used on whole yeast cells to induce mutations in exposed single-stranded regions in vivo. To understand the effects of bisulfite on live cells we conducted a genome-wide screen for bisulfite sensitive mutants in yeast. Screening the deletion mutant array, and collections of essential gene mutants we define a genetic network of bisulfite sensitive mutants. Validation of screen hits revealed hyper-sensitivity of transcription and RNA processing mutants, rather than DNA repair pathways and follow-up analyses support a role in perturbation of RNA transactions. We propose a model in which bisulfite-modified nucleotides may interfere with transcription or RNA metabolism when used in vivo.

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