Genome-wide bisulfite sensitivity profiling of yeast suggests bisulfite inhibits transcription.

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Mutation research, ISSN: 1873-135X, Vol: 821, Page: 13-19

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Segovia, Romulo; Mathew, Veena; Tam, Annie S; Stirling, Peter C
Elsevier BV
Biochemistry, Genetics and Molecular Biology; Environmental Science
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article description
Bisulfite, in the form of sodium bisulfite or metabisulfite, is used commercially as a food preservative. Bisulfite is used in the laboratory as a single-stranded DNA mutagen in epigenomic analyses of DNA methylation. Recently it has also been used on whole yeast cells to induce mutations in exposed single-stranded regions in vivo. To understand the effects of bisulfite on live cells we conducted a genome-wide screen for bisulfite sensitive mutants in yeast. Screening the deletion mutant array, and collections of essential gene mutants we define a genetic network of bisulfite sensitive mutants. Validation of screen hits revealed hyper-sensitivity of transcription and RNA processing mutants, rather than DNA repair pathways and follow-up analyses support a role in perturbation of RNA transactions. We propose a model in which bisulfite-modified nucleotides may interfere with transcription or RNA metabolism when used in vivo.