Hi-C 2.0: An optimized Hi-C procedure for high-resolution genome-wide mapping of chromosome conformation.

Citation data:

Methods (San Diego, Calif.), ISSN: 1095-9130, Vol: 123, Page: 56-65

Publication Year:
Usage 12
Abstract Views 11
Link-outs 1
Captures 4
Readers 4
Social Media 240
Shares, Likes & Comments 193
Tweets 47
Citations 2
Citation Indexes 2
Repository URL:
Belaghzal, Houda; Dekker, Job; Gibcus, Johan H.
Elsevier BV
Biochemistry, Genetics and Molecular Biology; Biochemistry, Biophysics, and Structural Biology; Computational Biology; Systems Biology; Chromosome conformation capture; Hi-C; Paired-end sequencing
Most Recent Tweet View All Tweets
article description
Chromosome conformation capture-based methods such as Hi-C have become mainstream techniques for the study of the 3D organization of genomes. These methods convert chromatin interactions reflecting topological chromatin structures into digital information (counts of pair-wise interactions). Here, we describe an updated protocol for Hi-C (Hi-C 2.0) that integrates recent improvements into a single protocol for efficient and high-resolution capture of chromatin interactions. This protocol combines chromatin digestion and frequently cutting enzymes to obtain kilobase (kb) resolution. It also includes steps to reduce random ligation and the generation of uninformative molecules, such as unligated ends, to improve the amount of valid intra-chromosomal read pairs. This protocol allows for obtaining information on conformational structures such as compartment and topologically associating domains, as well as high-resolution conformational features such as DNA loops.