Near-Infrared Intraoperative Chemiluminescence Imaging.

Citation data:

ChemMedChem, ISSN: 1860-7187, Vol: 11, Issue: 18, Page: 1978-82

Publication Year:
2016
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Repository URL:
http://hdl.handle.net/10754/621683
PMID:
27471800
DOI:
10.1002/cmdc.201600301
PMCID:
PMC5061038
Author(s):
Büchel, Gabriel E.; Carney, Brandon; Shaffer, Travis M.; Tang, Jun; Austin, Christine; Arora, Manish; Zeglis, Brian M.; Grimm, Jan; Eppinger, Jörg; Reiner, Thomas
Publisher(s):
Wiley-Blackwell
Tags:
Biochemistry, Genetics and Molecular Biology; Pharmacology, Toxicology and Pharmaceutics; Chemistry; Chemiluminescence
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article description
Intraoperative imaging technologies recently entered the operating room, and their implementation is revolutionizing how physicians plan, monitor, and perform surgical interventions. In this work, we present a novel surgical imaging reporter system: intraoperative chemiluminescence imaging (ICI). To this end, we have leveraged the ability of a chemiluminescent metal complex to generate near-infrared light upon exposure to an aqueous solution of Ce(4+) in the presence of reducing tissue or blood components. An optical camera spatially resolves the resulting photon flux. We describe the construction and application of a prototype imaging setup, which achieves a detection limit as low as 6.9 pmol cm(-2) of the transition-metal-based ICI agent. As a proof of concept, we use ICI for the in vivo detection of our transition metal tracer following both systemic and subdermal injections. The very high signal-to-noise ratios make ICI an interesting candidate for the development of new intraoperative imaging technologies.