Diversity of G proteins in lepidopteran cell lines: Partial sequences of six G protein alpha subunits
Archives of Insect Biochemistry and Physiology, ISSN: 0739-4462, Vol: 57, Issue: 3, Page: 142-150
2004
- 13Citations
- 10Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations13
- Citation Indexes13
- CrossRef13
- 13
- Captures10
- Readers10
- 10
Article Description
The aim of this work was to sample the diversity of G protein α subunits in lepidopteran insect cell lines. Here we report the amplification by degenerate PCR of partial sequences representing six G protein α subunits from three different lepidopteran insect cell lines. Sequence comparisons with known G protein α subunits indicate that the Sf9, Ld and High Five™ cell lines each contain (at least) one Gα-like and one Gα-like Gα subunit. All six PCR products are unique at the nucleotide level, but the translation products of the three Gα q-like partial clones (Sf9-Gα 1, Ld-Gα 1, and Hi5-Gα 1) are identical, as are the translation products of the three Gα i-like partial clones (Sf9-Gα 2, Ld-Gα 2, and Hi5-Gα 2). Both the Gα-like and Gα-like translation products are identical to known Gα subunits from other Lepidoptera, are highly similar (88-98%) to Gα subunits from other invertebrates including mosquitoes, fruit flies, lobsters, crabs, and snails, and are also highly similar (88-90%) to known mammalian Gα subunits. Identification of G protein α subunits in lepidopteran cell lines will assist in host cell line selection when insect cell lines are used for the pharmacological analysis of human GPCRs. © 2004 Wiley-Liss, Inc.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=8744316984&origin=inward; http://dx.doi.org/10.1002/arch.20018; http://www.ncbi.nlm.nih.gov/pubmed/15484257; https://onlinelibrary.wiley.com/doi/10.1002/arch.20018; http://doi.wiley.com/10.1002/arch.20018; https://onlinelibrary.wiley.com/doi/abs/10.1002/arch.20018
Wiley
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know