Evaluation of the Diagnostic Performance of the Xpert Clostridium difficile Assay and Its Comparison With the Toxin A/B Enzyme-Linked Fluorescent Assay and In-House Real-Time PCR Assay Used for the Detection of Toxigenic C. difficile
Journal of Clinical Laboratory Analysis, ISSN: 1098-2825, Vol: 28, Issue: 2, Page: 124-129
2014
- 7Citations
- 24Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations7
- Citation Indexes6
- CrossRef5
- Policy Citations1
- Policy Citation1
- Captures24
- Readers24
- 24
Article Description
Background: Clostridium difficile genes or toxin can be detected using several laboratory techniques. In this study, we compared the performance of the Xpert C. difficile assay with that of a toxin A/B enzyme-linked fluorescent immunoassay (ELFA) and an in-house real-time PCR assay for the tcdB gene. Methods: From April 2011 through January 2012, 138 soft or liquid stool samples from 138 adult patients at Paik Hospital were tested using the toxin A/B ELFA, in-house real-time PCR assay, and Xpert C. difficile assay to detect toxigenic C. difficile. Specimens were considered true positives if results were positive in both the in-house real-time PCR for tcdB gene and Xpert C. difficile assays. Results: Sensitivity of the toxin A/B ELFA, in-house tcdB gene real-time PCR, and Xpert C. difficile assay were 67.6%, 97.3%, and 100.0%, respectively. The specificity of the in-house tcdB gene real-time PCR assay was 100%, while the specificity was 98.0% for the other two methods. The turnaround time (TAT) was 50 min for the Xpert C. difficile assay, 75 min for the toxin A/B ELFA, and 160 min for the in-house real-time PCR assay. Conclusion: The Xpert C. difficile assay and the in-house real-time PCR assay had higher sensitivity than the toxin A/B ELFA; however, the specificities of the three assays were similar. Considering its rapid TAT and high sensitivity, use of the Xpert C. difficile assay is highly recommended for rapid and accurate diagnosis of C. difficile infection. © 2014 Wiley Periodicals, Inc.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84896109966&origin=inward; http://dx.doi.org/10.1002/jcla.21655; http://www.ncbi.nlm.nih.gov/pubmed/24395702; https://onlinelibrary.wiley.com/doi/10.1002/jcla.21655; https://dx.doi.org/10.1002/jcla.21655; https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.21655
Wiley
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