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Demonstration of 1α,25‐dihydroxyvitamin D receptor‐like molecule in ST 13 and 3T3 L1 preadipocytes and its inhibitory effects on preadipocyte differentiation

Journal of Cellular Physiology, ISSN: 1097-4652, Vol: 135, Issue: 3, Page: 545-550
1988
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The active metabolite of vitamin D, 1α,25‐dihydroxyvitamin D (1,25(OH)D), inhibited morphologic and enzymatic expression during differentiation of preadipocyte to adipocyte. In the presence of ∼6.4–20 × 10 M 1,25(OH)D, the triacylglycerol accumulation was only 50% of that of fully differentiated control cells. High‐affinity binding sites for 1,25(OH)D binding component sediments at 3.3 S in 4–24% (w/v) sucrose gradients prepared in hypertonic buffer. Binding assay revealed that N was 70 fmol/mg protein and 90 fmol/mg protein, and K value was 170 pM and 37 pM in cell lines ST 13 and 3T3 L1, respectively. We also found that differentiated adipocytes did not contain specific receptors for 1,25(OH)D. 1,25(OH)D, 1(OH)D, 24,25(OH)D, and 24(OH)D all suppressed differentiation of preadipocytes to adipocytes, and the dose required closely reflected the affinities of the various metabolites and the synthetic derivative for 1,25(OH)D receptor. It is suggested that the action of vitamin D on preadipocyte differentiation may result from a receptor‐mediated event. Copyright © 1988 Wiley‐Liss, Inc.

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