A Continuous Fluorescence Assay for Tryptophan Hydroxylase
Analytical Biochemistry, ISSN: 0003-2697, Vol: 266, Issue: 1, Page: 148-152
1999
- 25Citations
- 44Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations25
- Citation Indexes25
- 25
- CrossRef17
- Captures44
- Readers44
- 44
Article Description
A continuous fluorometric assay for tryptophan hydroxylase activity based on the different spectral characteristics of tryptophan and 5-hydroxytryptophan is presented. Hydroxylation of tryptophan at the 5-position results in a large increase in the fluorescence of the molecule. The assay selectively monitors the fluorescence yield of 5-hydroxytryptophan by exciting the reaction mix at 300 nm. The rate of increase of the emission signal was found to be directly proportional to the enzyme concentration. Inner filter effects due to quinonoid dihydropterin accumulation were eliminated by the inclusion of a thiol reductant. Activity measured using this assay method was found to be the same as that determined by established discontinuous HPLC assay methods. The application of the assay to routine activity measurements and to steady-state determinations with the substrates tryptophan and tetrahydropterin is described.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0003269798929566; http://dx.doi.org/10.1006/abio.1998.2956; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0032955631&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/9887224; https://linkinghub.elsevier.com/retrieve/pii/S0003269798929566; https://dx.doi.org/10.1006/abio.1998.2956
Elsevier BV
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