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Microdissection and visualization of individual hair follicles for lineage tracing studies.

Methods in molecular biology (Clifton, N.J.), ISSN: 1940-6029, Vol: 1195, Page: 247-58
2014
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Article Description

In vivo lineage tracing is a valuable technique to study cellular behavior. Our lab developed a lineage tracing method, based on the Cre/lox system, to genetically induce clonal labelling of cells and follow their progeny. Here we describe a protocol for temporally controlled clonal labelling and for microdissection of individual mouse hair follicles. We further present staining and visualization techniques used in our lab to analyze clones issued from genetically induced labelling.

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