Effective Transfection and Gene Silencing of Primary Murine Macrophages with Small Interfering RNA
Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2419, Page: 125-132
2022
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
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Book Chapter Description
Transfection of murine primary macrophages to silence genes can be a challenging procedure because this cell type has developed mechanisms to evade cellular intrusion. The introduction of small interfering RNA (siRNA) encapsulated in liposomes to the cell to decrease gene expression is one of the methods that can be used to achieve gene silencing. There are different commercially available compounds to introduce siRNA into the cell, including Lipofectamine RNAiMAX and HiPerfect. The chapter will describe a method for gene silencing in mouse primary macrophages using liposome-based transfection of siRNA.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85125614528&origin=inward; http://dx.doi.org/10.1007/978-1-0716-1924-7_8; http://www.ncbi.nlm.nih.gov/pubmed/35237962; https://link.springer.com/10.1007/978-1-0716-1924-7_8; https://dx.doi.org/10.1007/978-1-0716-1924-7_8; https://link.springer.com/protocol/10.1007/978-1-0716-1924-7_8
Springer Science and Business Media LLC
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