Measurement of Cytosolic Mitochondrial DNA After NLRP3 Inflammasome Activation
Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2459, Page: 117-129
2022
- 2Citations
- 6Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations2
- Citation Indexes2
- Captures6
- Readers6
Book Chapter Description
The NLRP3 inflammasome, a key component of the innate immune system that mediates caspase-1 activation, which in turn induces cleavage of the pyroptosis executioner gasdermin D and the proinflammatory cytokines IL-1β and IL-18, requires two signals to be activated. First, inflammasome priming is achieved after activation of Toll-like receptors, which leads to NF-κB signaling and transcriptional activation of the genes for NLRP3 and IL-1β. Next, the inflammasome complex is activated by a second signal that induces extrusion of mitochondrial DNA to the cytosol of the cell, which leads to its oligomerization by a not fully understood mechanism. Here we describe a simple method that employs quantitative polymerase chain reaction (qPCR) using SYBR green to measure the presence of mitochondrial DNA (mtDNA) in the cytosol, which can be used to measure cytosolic mtDNA levels after inflammasome activation.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85125548573&origin=inward; http://dx.doi.org/10.1007/978-1-0716-2144-8_12; http://www.ncbi.nlm.nih.gov/pubmed/35212960; https://link.springer.com/10.1007/978-1-0716-2144-8_12; https://dx.doi.org/10.1007/978-1-0716-2144-8_12; https://link.springer.com/protocol/10.1007/978-1-0716-2144-8_12
Springer Science and Business Media LLC
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