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Base Editing of Human Hematopoietic Stem Cells

Methods in Molecular Biology, ISSN: 1940-6029, Vol: 2606, Page: 43-62
2023
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Book Chapter Description

Base editing by nucleotide deaminases linked to programmable DNA-binding proteins represents a promising approach to remedy blood disorders. Here we describe the ex vivo base editing of human CD34+ hematopoietic stem and progenitor cells (HSPCs) by electroporation of base editor mRNA or protein.

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