Detection and automated analysis of single transcripts at subcellular resolution in zebrafish embryos
Methods in Molecular Biology, ISSN: 1064-3745, Vol: 1649, Page: 143-162
2018
- 5Citations
- 11Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations5
- Citation Indexes5
- Captures11
- Readers11
- 11
Book Chapter Description
Single molecule fluorescence in situ hybridization (smFISH) is a method to visualize single mRNA molecules. When combined with cellular and nuclear segmentation, transcripts can be assigned to different cellular compartments resulting in quantitative information on transcript levels at subcellular resolution. The use of smFISH in zebrafish has been limited by the lack of protocols and an automated image analysis pipeline for samples of multicellular organisms. Here we present a protocol for smFISH on zebrafish cryosections. The protocol includes a method to obtain high-quality sections of zebrafish embryos, an smFISH protocol optimized for zebrafish cryosections, and a user-friendly, automated analysis pipeline for cell segmentation and transcript detection. The software is freely available and can be used to analyze sections of any multicellular organism.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85033662626&origin=inward; http://dx.doi.org/10.1007/978-1-4939-7213-5_9; http://www.ncbi.nlm.nih.gov/pubmed/29130195; http://link.springer.com/10.1007/978-1-4939-7213-5_9; https://dx.doi.org/10.1007/978-1-4939-7213-5_9; https://link.springer.com/protocol/10.1007/978-1-4939-7213-5_9
Springer Nature America, Inc
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