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Mitochondrial Isolation and Real-Time Monitoring of MOMP

Methods in Molecular Biology, ISSN: 1940-6029, Vol: 1877, Page: 121-130
2019
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Book Chapter Description

Isolated model systems have proven to be the standard in the apoptosis field to deconstruct MOMP into individual steps and to study the behavior of a subset of MOMP regulators. Here we describe the method to isolate, JC-1-label, and purify mouse liver mitochondria and subsequently describe how to utilize the JC-1-labeled mitochondria for real-time MOMP measurements.

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