Sequence of a cellulase gene from the rumen anaerobe Ruminococcus flavefaciens 17
MGG Molecular & General Genetics, ISSN: 0026-8925, Vol: 228, Issue: 1-2, Page: 320-323
1991
- 20Citations
- 6Captures
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Metrics Details
- Citations20
- Citation Indexes20
- 20
- CrossRef15
- Captures6
- Readers6
Article Description
A cellulase gene (endA) was isolated from a library of Ruminococcus flavefaciens strain 17 DNA fragments inserted in pUC13. The endA product showed activity against acid-swollen cellulose, carboxymethyl-cellulose, lichenan, cellopentaose and cellotetraose, but showed no activity against cellotriose or binding to avicel. Nucleotide sequencing indicated an encoded product of 455 amino acids which showed significant sequence similarity (ranging from 56% to 61%) with three endoglucanases from Ruminococcus albus, and with Clostridium thermocellum endoglucanase E. Little relatedness was found with a cellodextrinase previously isolated from R. flavefaciens FD1. © 1991 Springer-Verlag.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0025733993&origin=inward; http://dx.doi.org/10.1007/bf00282484; http://www.ncbi.nlm.nih.gov/pubmed/1886616; http://link.springer.com/10.1007/BF00282484; http://www.springerlink.com/index/10.1007/BF00282484; http://www.springerlink.com/index/pdf/10.1007/BF00282484; https://dx.doi.org/10.1007/bf00282484; https://link.springer.com/article/10.1007/BF00282484
Springer Science and Business Media LLC
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