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Untersuchungen zur Argentaffinreaktion des Nukleolus

Zeitschrift für Zellforschung und Mikroskopische Anatomie, ISSN: 0302-766X, Vol: 76, Issue: 4, Page: 577-605
1967
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Article Description

A silver impregnation method was applied to human tumor cells of the HeLa strain growing in vitro. Preparations of the same series were examined with both light and electron microscopes. The following results were obtained with respect to the specific affinity for silver of the filamentous structures of the nucleoli, which have been designated "nucleolonema" by Estable and Sotelo: 1. The silver reaction depends on the particular fixative used. 2. After fixation with OsO some of the components of the nucleolus are clearly distinguishable with the electron microscope, apparently as a result of extraction of certain constituents of the nucleolus during the impregnation process. Silver grains are deposited on the surface of chromosomal fibrillae both inside and outside the nucleoli. 3. After formaldehyde or glutaraldehyde fixation rosary-like structures are visible within the nucleolus, when viewed with the light microscope. Dense aggregation of the filaments, which are here and there strongly impregnated with silver, gives the impression of a continuous deposition of silver along these filamentous structures. It is evident from electron microscope examination, however, that the areas with an affinity for silver, which is discontinuously distributed along the filaments, are actually parts of a filamentous structure. 4. This observation of a filamentous structure is confirmed in cultures pretreated with adenosine after which there is an "unfolding" of the filamentous structures. They thus become visible over longer distances, as shown by both light and electron microscopes. We conclude that the "nucleolonema" is a complex structure made up of different components, only part of which may be identifiable after a given technique. The following components have been demonstrated: deoxyribonucleoprotein, ribonucleoprotein, and a component reacting under special conditions with silver, which may be a lipoprotein. This interpretation of the "nucleolonema" as a complex structure reconciles certain here-to-fore seemingly-contradictory concepts of the nature of the "nucleolonema". © 1967 Springer-Verlag.

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