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Ion permeability of rabbit intestinal brush border membrane vesicles

The Journal of Membrane Biology, ISSN: 0022-2631, Vol: 78, Issue: 2, Page: 119-127
1984
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Article Description

The ion permeability of rabbit jejunal brush border membrane vesicles was studied by measuring unidirectional fluxes with radioactive tracers and bi-ionic diffusion potentials with the potential-sensitive fluorescent dye, diS-C-(5). Tracer measurements provide estimates of the absolute magnitudes of permeability coefficients, while fluorescence measurements provide estimates of relative and absolute ion permeabilities. The magnitudes of the permeability coefficients for Na, K, Rb, and Br were approximately 5 nanoliters/(mg protein × sec) or 10 cm/sec as determined by radioactive tracer measurements. The apparent selectivity sequence, relative to Na, as determined by bi-ionic potential measurements was: F, isetheionate, gluconate, choline (<0.1)

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