Alginate immobilization of recombinant Escherichia coli whole cells harboring l-arabinose isomerase for l-ribulose production
Bioprocess and Biosystems Engineering, ISSN: 1615-7591, Vol: 33, Issue: 6, Page: 741-748
2010
- 52Citations
- 54Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations52
- Citation Indexes52
- 52
- CrossRef48
- Captures54
- Readers54
- 54
Article Description
Recombinant Escherichia coli whole cells harboring Bacillus licheniformis l-arabinose isomerase (BLAI) were immobilized with alginate. The operational conditions for immobilization were optimized with response surface methodology. Optimal alginate concentration, Ca concentration, and cell mass loading were 1.8% (w/v), 0.1 M, and 44.5 g L, respectively. The interactions between Ca concentration, alginate concentration, and initial cell mass were significant. After immobilization of BLAI, cross-linking with 0.1% glutaraldehyde significantly reduced cell leakage. The half-life of immobilized whole cells was 150 days, which was 50-fold longer than that of free cells. In seven repeated batches for l-ribulose production, the productivity was as high as 56.7 g L h at 400 g L substrate concentration. The immobilized cells retained 89% of the initial yield after 33 days of reaction. Immobilization of whole cells harboring BLAI, therefore, makes a suitable biocatalyst for the production of l-ribulose, particularly because of its high stability and low cost. © 2009 Springer-Verlag.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77955519658&origin=inward; http://dx.doi.org/10.1007/s00449-009-0397-7; http://www.ncbi.nlm.nih.gov/pubmed/19946709; http://link.springer.com/10.1007/s00449-009-0397-7; http://www.springerlink.com/index/10.1007/s00449-009-0397-7; http://www.springerlink.com/index/pdf/10.1007/s00449-009-0397-7; https://dx.doi.org/10.1007/s00449-009-0397-7; https://link.springer.com/article/10.1007/s00449-009-0397-7
Springer Science and Business Media LLC
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