Simultaneous electrochemical determination of ochratoxin A and fumonisin B1 with an aptasensor based on the use of a Y-shaped DNA structure on gold nanorods
Microchimica Acta, ISSN: 1436-5073, Vol: 187, Issue: 2, Page: 102
2020
- 43Citations
- 33Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations43
- Citation Indexes43
- 43
- CrossRef6
- Captures33
- Readers33
- 33
Article Description
A complementary DNA (cDNA) was designed to simultaneously hybridize with the ochratoxin A (OTA) aptamer and the fumonisin B1 (FB1) aptamer to form a unique Y-shaped DNA structure and to achieve simultaneous detection. Gold nanorods (AuNRs) were used to immobilize thionine (Th), thiolated ferrocene (Fc), thiolated OTA aptamer (Apt1), and thiolated FB1 aptamer (Apt2), to form an amplified signal element and a recognition element. The Apt1-AuNRs-Th complex and the Apt2-AuNRs-Fc complex hybridize with cDNA to form a unique Y-DNA structure on a gold electrode. This produces two initial electrochemical signals [with 177 μΑ cm near −0.2 V, and 3121 μΑ cm near +0.46 V (vs. Ag/AgCl)] by differential pulse voltammetry. Upon addition of 0.1 ng mL OTA and 0.1 ng mL FB1, the aptamers bind the two toxins. This results in the release of Apt1-AuNRs-Th and Apt2-AuNRs-Fc, so the peak currents densities decrease to 115 μΑ cm and 209 μΑ cm. The assay allows simultaneous determination of OTA and FB1 in the 1.0 pg·mL to 100 ng·mL concentration ranges, with LODs of 0.47 and 0.26 pg·mL. The assay is reproducible, stable and specific. It was applied to the determination of OTA and FB1 in spiked beer, with recoveries between 89.0% and 102.0%. [Figure not available: see fulltext.].
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85077612652&origin=inward; http://dx.doi.org/10.1007/s00604-019-4089-y; http://www.ncbi.nlm.nih.gov/pubmed/31912309; http://link.springer.com/10.1007/s00604-019-4089-y; https://dx.doi.org/10.1007/s00604-019-4089-y; https://link.springer.com/article/10.1007/s00604-019-4089-y
Springer Science and Business Media LLC
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