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Molecular cloning and expression of amylosucrase from highly radiation-resistant Deinococcus radiopugnans

Food Science and Biotechnology, ISSN: 2092-6456, Vol: 23, Issue: 6, Page: 2007-2012
2014
  • 24
    Citations
  • 0
    Usage
  • 9
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    24
    • Citation Indexes
      24
  • Captures
    9

Article Description

Amylosucrase (AS), a glucosyltransferase of the glycoside hydrolase 13 family, does not require a nucleotide-activated sugar as a glucosyl-donor. A gene (drpas) of Deinococcus radiopugnans encoding a putative AS was identified and cloned for characterization. The amino acid sequence revealed that drpas exhibited 76 and 74% identities with AS genes from D. geothermalis and D. radiodurans, respectively. Recombinant DRpAS exhibited AS activities. The ratios of hydrolysis, polymerization, and isomerization reactions were 5.8:82.7:11.5 at 40°C. The DRpAS was highly thermostable and produced more polymerization products than AS from D. radiodurans although the optimum growth temperature of both strains is 30°C.

Bibliographic Details

Myo Deok Kim; Dong Ho Seo; Jong Hyun Jung; Dong Hyun Jung; Ju Hoon Lee; Cheon Seok Park; Min Ho Joe; Sangyong Lim

Springer Science and Business Media LLC

Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; Immunology and Microbiology

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