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Genetic diversity of 3′ region of glycoprotein D gene of bovine herpesvirus 1 and 5

Virus Genes, ISSN: 1572-994X, Vol: 48, Issue: 3, Page: 438-447
2014
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Article Description

Bovine herpesviruses 1 (BoHV-1) and 5 (BoHV-5) are closely related alphaherpesviruses of cattle. While BoHV-1 is mainly associated with respiratory/genital disease and rarely associated with neurological disease, BoHV-5 is the primary agent of meningoencephalitis in cattle. The envelope glycoprotein D of alphaherpesviruses (BoHV-1/gD1 and BoHV-5/gD5) is involved in the early steps of virus infection and may influence virus tropism and neuropathogenesis. This study performed a sequence analysis of the 3′ region of gD gene (gD3′) of BoHV-1 isolates recovered from respiratory/genital disease (n = 6 and reference strain Cooper) or from neurological disease (n = 7); and from seven typical neurological BoHV-5 isolates. After PCR amplification, nucleotide (nt) sequencing, and aminoacid (aa) sequence prediction; gD3′ sequences were compared, identity levels were calculated, and selective pressure was analyzed. The phylogenetic reconstruction based on nt and aa sequences allowed for a clear differentiation of BoHV-1 (n = 14) and BoHV-5 (n = 7) clusters. The seven BoHV-1 isolates from neurological disease are grouped within the BoHV-1 branch. A consistent alignment of 346 nt revealed a high similarity within each viral species (gD1 = 98.3% nt and aa; gD5 = 97.8% nt and 85.8% aa) and an expected lower similarity between gD1 and gD5 (73.7 and 64.1%, nt and aa, respectively). The analysis of molecular evolution revealed an average negative selection at gD3′. Thus, the phylogeny and similarity levels allowed for differentiation of BoHV-1 and BoHV-5 species, but not further division in subspecies. Sequence analysis did not allow for the identification of genetic differences in gD3′ potentially associated with the respective clinical/pathological phenotypes, yet revealed a lower level of gD3′ conservation than previously reported. © Springer Science+Business Media 2014.

Bibliographic Details

Traesel, Carolina Kist; Sá e Silva, Mariana; Weiss, Marcelo; Spilki, Fernando Rosado; Weiblen, Rudi; Flores, Eduardo Furtado

Springer Science and Business Media LLC

Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology

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