Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus.

Citation data:

Methods in molecular biology (Clifton, N.J.), ISSN: 1940-6029, Vol: 1604, Page: 179-188

Publication Year:
2018
PMID:
28986833
DOI:
10.1007/978-1-4939-6981-4_12
Author(s):
Qi, Xiaoxuan; Wang, Wenjian; Dong, Haohao; Liang, Yuying; Dong, Changjiang; Ly, Hinh
Publisher(s):
Springer Nature
Tags:
Biochemistry, Genetics and Molecular Biology
book chapter description
We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at a resolution of 2.36 Å. The crystals belong to space group P3, with the unit cell parameters a = b = 176.35 Å, c = 56.40 Å, α = β = 90°, and γ = 120°. Using the X-ray diffraction method, we constructed a three-dimensional structure of the LASV NP that should aid in the development of novel therapeutic strategies against this virus, for which vaccine and effective treatment modalities are currently unavailable.