PMID:
29170957
DOI:
10.1007/978-1-4939-7295-1_9
Author(s):
Hsu, Szu-Yi, Smanski, Michael J
Publisher(s):
Springer Nature
Tags:
Biochemistry, Genetics and Molecular Biology
book chapter description
Advances in DNA synthesis and assembly technology allow for the high-throughput fabrication of hundreds to thousands of multi-part genetic constructs in a short time. This allows for rapid hypothesis-testing and genetic optimization in multi-gene biological systems. Here, we discuss key considerations to design and implement an algorithmic DNA assembly pipeline that provides the freedom to change nearly any design variable in a multi-gene system. In addition to considerations for pipeline design, we describe protocols for three useful molecular biology techniques in plasmid construction.

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