Designing and Implementing Algorithmic DNA Assembly Pipelines for Multi-Gene Systems.
- Citation data:
Methods in molecular biology (Clifton, N.J.), ISSN: 1940-6029, Vol: 1671, Page: 131-147
- Publication Year:
- Captures 6
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- Biochemistry, Genetics and Molecular Biology
book chapter description
Advances in DNA synthesis and assembly technology allow for the high-throughput fabrication of hundreds to thousands of multi-part genetic constructs in a short time. This allows for rapid hypothesis-testing and genetic optimization in multi-gene biological systems. Here, we discuss key considerations to design and implement an algorithmic DNA assembly pipeline that provides the freedom to change nearly any design variable in a multi-gene system. In addition to considerations for pipeline design, we describe protocols for three useful molecular biology techniques in plasmid construction.