Designing and Implementing Algorithmic DNA Assembly Pipelines for Multi-Gene Systems.

Citation data:

Methods in molecular biology (Clifton, N.J.), ISSN: 1940-6029, Vol: 1671, Page: 131-147

Publication Year:
2018
Captures 6
Readers 6
PMID:
29170957
DOI:
10.1007/978-1-4939-7295-1_9
Author(s):
Hsu, Szu-Yi; Smanski, Michael J
Publisher(s):
Springer Nature
Tags:
Biochemistry, Genetics and Molecular Biology
book chapter description
Advances in DNA synthesis and assembly technology allow for the high-throughput fabrication of hundreds to thousands of multi-part genetic constructs in a short time. This allows for rapid hypothesis-testing and genetic optimization in multi-gene biological systems. Here, we discuss key considerations to design and implement an algorithmic DNA assembly pipeline that provides the freedom to change nearly any design variable in a multi-gene system. In addition to considerations for pipeline design, we describe protocols for three useful molecular biology techniques in plasmid construction.