Experimental Aspects of Polarization Optimized Experiments (POE) for Magic Angle Spinning Solid-State NMR of Microcrystalline and Membrane-Bound Proteins.

Citation data:

Methods in molecular biology (Clifton, N.J.), ISSN: 1940-6029, Vol: 1688, Page: 37-53

Publication Year:
2018
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PMID:
29151203
DOI:
10.1007/978-1-4939-7386-6_2
Author(s):
Gopinath, T; Veglia, Gianluigi
Publisher(s):
Springer Nature
Tags:
Biochemistry, Genetics and Molecular Biology
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book chapter description
Conventional NMR pulse sequences record one spectrum per experiment, while spending most of the time waiting for the spin system to return to the equilibrium. As a result, a full set of multidimensional NMR experiments for biological macromolecules may take up to several months to complete. Here, we present a practical guide for setting up a new class of MAS solid-state NMR experiments (POE or polarization optimized experiments) that enable the simultaneous acquisition of multiple spectra of proteins, accelerating data acquisition. POE exploit the long-lived N polarization of isotopically labeled proteins and enable one to obtain up to eight spectra, by concatenating classical NMR pulse sequences. This new strategy propels data throughput of solid-state NMR spectroscopy of fibers, microcrystalline preparations, as well as membrane proteins.