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Escherichia coli Integration Host Factor inhibits the NusA stimulation of RNA polymerase sigma subunit synthesis in vitro

Archives of Biochemistry and Biophysics, ISSN: 0003-9861, Vol: 243, Issue: 1, Page: 315-319
1985
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As reported previously, Integration Host Factor (IHF) stimulates cII expression but the stimulatory effect is prevented by the NusA protein ( Peacock and Weissbach, 1985, Biochem. Biophys. Res. Commun. 127, 1026–1031). The interaction between IHF and the NusA protein has been investigated further in studies on the in vitro expression of the genes for the β ( rpoB ) and σ ( rpoD ) subunits of RNA polymerase, both known to be stimulated by NusA. The NusA stimulation of rpoD expression can be prevented by IHF, but IHF has no effect by itself on rpoD expression. IHF does not influence rpoB expression either in the presence or absence of NusA.

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