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Human anti-F(ab′) 2 antibodies show preferential reactivity for F(ab′) 2 molecules bearing λ light chains

Clinical Immunology and Immunopathology, ISSN: 0090-1229, Vol: 65, Issue: 2, Page: 176-182
1992
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Article Description

In order to evaluate binding specificities of anti-F(ab′) 2 antibodies from patients with systemic lupus erythematosus (SLE) and from normal healthy controls, F(ab′) 2 fragments were prepared from 24 IgG myelomas with defined isoelectric points, DNA-associated idiotypes, and κ/λ light chain types. Using ELISA and hemagglutination assays, anti-F(ab′) 2 antibodies from 12 healthy controls and 29 SLE patients were observed to exhibit preferential binding (λ > κ) to myeloma F(ab′) 2 fragments composed of λ light chains ( P < 0.0001). No correlation of anti-F(ab′) 2 binding and presence of cationic, neutral, or anionic isoelectric points or for DNA-associated idiotypes on monoclonal F(ab′) 2 was detected. Anti-F(ab′) 2 antibodies, often elevated in SLE during remission, show preferential specificity for F(ab′) 2 fragments bearing λ light chains.

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