Urinary excretion of free glucocorticosteriods and testosterone in the Mongolian gerbil ( Meriones unguiculatus ): Effects of long-acting corticotrophin and human gonadotrophin
Comparative Biochemistry and Physiology Part A: Physiology, ISSN: 0300-9629, Vol: 91, Issue: 4, Page: 789-795
1988
- 14Citations
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Metrics Details
- Citations14
- Citation Indexes14
- 14
- CrossRef8
Article Description
1.1. Methods for the quantitative collection of 24-hr urines of small laboratory animals and for the measurement of urinary free glucocorticosteroids and testosterone are described.2.2. Urinary glucocorticosteroids and testosterone were determined in 0.1–0.5 ml-aliquots of 1100 diluted urines after kieselgur mini-column extraction.3.3. Excretion of glucocorticosteroids and testosterone in undisturbed Mongolian gerbils was 329 and 13 ng/day, respectively.4.4. Administration of long-acting (1–24)ACTH (20 IU/animal) increased glucocorticosteroid and testosterone excretion to about 2000 ng/day (glucocorticosteriods) and to about 30 ng/day (testosterone) over 3 days.5.5. In animals injected with 100 IU/animal HCG, testosterone excretion was elevated to about 35–50 ng/day over 3 days.6.6. As the results show, the measurement of urinary excretion of free glucocorticosteroids and testosterone is a relable index of adrenal-gonadal function in the Mongolian gerbil.7.7. Furthermore, in small laboratory animals, steroid measurements in 24-hr urines may be superior to determinations in plasma, since amounts of urinary steroid are relatively high and 24-hr urines can be collected over longer time periods without stressing the animals.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/0300962988909668; http://dx.doi.org/10.1016/0300-9629(88)90966-8; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0023771472&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/2907447; https://linkinghub.elsevier.com/retrieve/pii/0300962988909668; http://dx.doi.org/10.1016/0300-9629%2888%2990966-8; https://dx.doi.org/10.1016/0300-9629%2888%2990966-8
Elsevier BV
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