Microinjection of oocytes and embryos with synthetic mRNA encoding molecular probes
Methods in Cell Biology, ISSN: 0091-679X, Vol: 150, Page: 189-222
2019
- 10Citations
- 13Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations10
- Citation Indexes10
- 10
- CrossRef6
- Captures13
- Readers13
- 13
Article Description
We describe methods and techniques for introduction of molecular probes in the form of synthetic mRNA by rapid repetitive microinjection into oocytes or early embryos of echinoderms and various invertebrates. Construct assembly is followed by standard kit-based in vitro mRNA synthesis, with slight modifications to optimize expression and clean-up. Variations of a basic microinjection procedures are detailed for echinoderms: starfish oocytes ( Patiria miniata or other species), purple urchin ( Strongylocentrotus purpuratus ) and sand dollar ( Dendraster excentricus ) zygotes, with notes included for other invertebrate eggs and embryos as well.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0091679X1830150X; http://dx.doi.org/10.1016/bs.mcb.2018.10.012; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85058640998&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/30777176; https://linkinghub.elsevier.com/retrieve/pii/S0091679X1830150X; https://dx.doi.org/10.1016/bs.mcb.2018.10.012
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know