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Early embryo-endometrial signaling modulates the regulation of matrix metalloproteinase-3

Fertility and Sterility, ISSN: 0015-0282, Vol: 82, Issue: SUPPL. 3, Page: 1029-1035
2004
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Article Description

To examine matrix metalloproteinase-3 (MMP-3) expression in human stromal cell culture after P stimulation and the effect of conditioned medium from human embryo-epithelial cells coculture on its expression and activity. Metabolic and endocrine studies on human tissue. In vitro fertilization (IVF) unit and endocrine research unit. Infertile patients undergoing endometrial tissue sampling for dating at the luteal phase before IVF. Endometrial sampling and collection of human embryos culture media. Expression and activity of secreted MMP-3 by P-induced stromal cells, and in stromal cells exposed to conditioned medium from embryo-epithelial cell coculture. Expression and activity of MMP-3 in human stromal cells decrease after P induction. Following incubation of these stromal-derived decidual cells with conditioned medium from embryo-epithelial cell coculture, MMP-3 expression and activity increased in a statistically significant manner. Progesterone inhibition of MMP-3 expression and its support of endometrial integrity were prevented by local expression of MMP-3 in response to embryonic signaling.

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