High prevalence of serological weak D phenotype and preponderance of weak D type 4.0.1. genetic variant in a Nigerian population: implications for transfusion practice in a resource-limited setting
Hematology, Transfusion and Cell Therapy, ISSN: 2531-1379, Vol: 44, Issue: 3, Page: 386-391
2022
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Article Description
Prevalence of RhD negative phenotype in Nigeria is low; this leads to scarcity of RhD negative red cells for transfusion. Serological and molecular genotyping of RhD negative individuals for weak D types could reduce this scarcity. The aim of this study was to determine the serological prevalence and molecular types of weak D phenotypes among blood donors and pregnant women in Kano, Nigeria. A total of 4482 blood donors and pregnant women from three hospitals in Kano were recruited. An indirect antiglobulin test was used to determine weak D phenotypes. Molecular genotyping was performed on genomic DNA from whole blood amplified by polymerase chain reaction sequence-specific primers (PCR-SSP) with agarose gel electrophoresis. The mean age of the participants was 26.50 ± 5.79 years. The prevalence of the RhD negative phenotype was 4.2% (189/4482). Of the 189 RhD negative phenotypes, 20 (10.6%) were weak D positive. Molecular genotyping of the 20 Weak D positive phenotypes revealed 15 (75%) weak D type 4, of which 11 were due to the RHD*09.03 and RHD*DAR3 (T201R, F223V) polymorphisms and 4, due to RHD* 08.01 and RHD* DFV polymorphisms; 2 (10%) were due to the 602 C>G polymorphism, while the remaining 3 (15%) constituted partial D or other rare weak D types. The prevalence of weak D positive phenotypes is high in this study; weak D type 4 is the most common RhD genetic variant. Routine serologic weak D testing of RhD negative blood and molecular genotyping should be encouraged in resource-limited settings.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S2531137921000365; http://dx.doi.org/10.1016/j.htct.2021.01.011; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85102833392&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/33744228; https://linkinghub.elsevier.com/retrieve/pii/S2531137921000365; http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2531-13792022000300386&lng=en&tlng=en; http://www.scielo.br/scielo.php?script=sci_abstract&pid=S2531-13792022000300386&lng=en&tlng=en; http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2531-13792022000300386; http://www.scielo.br/scielo.php?script=sci_abstract&pid=S2531-13792022000300386; https://dx.doi.org/10.1016/j.htct.2021.01.011
Elsevier BV
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