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Acetylation of ( R , S )-propranolol catalyzed by Candida antarctica lipase B: An experimental and computational study

Journal of Molecular Catalysis B: Enzymatic, ISSN: 1381-1177, Vol: 98, Page: 21-29
2013
  • 21
    Citations
  • 0
    Usage
  • 33
    Captures
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Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    21
    • Citation Indexes
      21
  • Captures
    33

Article Description

The chemo- and enantioselectivity of the Candida antarctica lipase B (CalB)-catalyzed acetylation reaction of ( R, S )-propranolol using vinyl acetate as acyl donor and toluene as organic solvent was studied. Because of the poor solubility of propranolol in toluene small quantities of methanol were added as cosolvent. The effects of the propranolol/vinyl acetate ratio, the enzyme purification procedure and the methanol concentration on the reaction were investigated. The reactions occurring in the system were quantitatively investigated using 1 H and 13 C NMR spectroscopy. The major reactions were the hydrolysis and alcoholysis of vinyl acetate, as a consequence of the presence of residual water and methanol in the reaction medium. Furthermore, the NMR analysis confirmed that O -acetyl-propranolol was formed exclusively. The reaction was also found to be enantioselective favoring the faster transformation of the R -propranolol. In addition to the experiments, molecular modeling was used to study the formation of the reactive Michaelis complexes between propranolol and acetylated CalB, using a combined molecular docking and molecular dynamics (MD) procedure. Only for the O -acetylation we found binding modes of the substrate leading to formation of the product, which explains the experimentally observed chemoselectivity of CalB.

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