Measures of diagnostic precision (repeatability and reproducibility) for three test methods designed to detect spring viremia of carp virus
Preventive Veterinary Medicine, ISSN: 0167-5877, Vol: 188, Page: 105288
2021
- 4Citations
- 13Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations4
- Citation Indexes4
- CrossRef1
- Captures13
- Readers13
- 13
Article Description
Spring viremia of carp virus (SVCV) is a rhabdovirus of the Sprivivirus genus and the etiological agent of an internationally regulated aquatic animal disease in several fish species, including koi carp Cyprinus carpio L. The virus has a complex lifecycle with both acute and persistent stages of infection and can cause high mortality in affected populations. In this study, the diagnostic repeatability (within laboratory agreement) and reproducibility (between laboratory agreement) of 3 tests were investigated to assess their fitness as SVCV diagnostic tools. The tests, reverse transcription quantitative polymerase chain reaction (RT-qPCR) assays targeting either the SVCV glycoprotein (Q1G) or nucleoprotein (Q2N) genes and virus isolation by cell culture (VI), were performed in a blinded study with four Canadian laboratories. Test panels consisted of duplicate sets of 100 tissue samples collected from 3 SVCV prevalence populations of koi: a low-prevalence negative reference population (n = 20 fish) as well as moderate- (n = 50 fish) and high-prevalence (n = 30 fish) populations of koi experimentally infected with SVCV. The Q1G and Q2N tests were performed with kidney tissue in 3 laboratories and with brain tissue in 1 laboratory whereas pools of kidney, spleen and gill tissues were tested with the VI assay in 2 laboratories. Agreement of binary results was evaluated using the observed proportion of agreement, Cohen’s kappa and Gwet’s agreement coefficient (AC1) whereas the concordance correlation coefficient (ccc) and Bland Altman’s limit of agreement were used to evaluate agreement of the RT-qPCR continuous data. Gwet’s AC1 provided a more stable estimate of agreement than Cohen’s kappa. Overall, high repeatability (AC1, 0.78−0.90) and reproducibility (AC1, 0.74−0.89) were observed for the Q1G and Q2N tests when kidney tissue was used. Lower agreement estimates of repeatability (AC1, 0.54−0.77) and reproducibility (AC1, 0.50−0.80) were obtained for the VI test. RT-qPCR reproducibility was low with kidney-brain tissue pairs (AC1, 0.09−0.46) and high with inter-test pairs of brain (AC1, 0.76−0.86) or kidney tissue (0.75−0.86). Tissue-specific differences in virus load affected test precision and informed final tissue selection. Repeatability (ccc, 0.94−0.97) and reproducibility (ccc, 0.91−0.97) estimates of agreement for paired continuous data from the RT-qPCR assays were similarly high with kidney tissue and lower with paired brain (ccc, 0.15−0.83) and kidney-brain tissues (ccc, 0.01−0.55). The high precision of Q1G and Q2N with kidney tissue suggests that the tests are performing similarly and are suitable candidates for assessment of their diagnostic accuracy.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0167587721000325; http://dx.doi.org/10.1016/j.prevetmed.2021.105288; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85100412514&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/33551191; https://linkinghub.elsevier.com/retrieve/pii/S0167587721000325; https://dx.doi.org/10.1016/j.prevetmed.2021.105288
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know