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DNA-functionalized metal-organic framework ratiometric nanoprobe for MicroRNA detection and imaging in live cells

Sensors and Actuators B: Chemical, ISSN: 0925-4005, Vol: 361, Page: 131676
2022
  • 38
    Citations
  • 0
    Usage
  • 9
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    38
    • Citation Indexes
      38
  • Captures
    9

Article Description

MicroRNAs are short noncoding regulatory RNAs that are increasingly used as disease biomarkers. Rapid, sensitive, and quantitative microRNAs detection methods with high specificity are imperative. Here, we for the first time developed a DNA-functionalized metal-organic framework (MOF)-based ratiometric fluorescent probe using the interaction between Thiazole Orange (TO) dye and Rhodamine B(RhB) dye molecules, and achieved sensitive detection and imaging of miRNA-21 in living cells. Among them, DNA was successfully conjugated with MOF through the Zr-O-P bond in a single step with high efficiency. Based on this ratiometric fluorescent probe, picomolar limit detection (57 pM) for miRNA-21 can be obtained without signal amplification. After penetration into the cell, intracellular miRNA-21 can also be imaged by strand-displacement reaction, even distinguishing cancer cells from normal cells was achieved. This work paves the way for the development of DNA-functionalized MOF for intracellular molecules analysis and is of great significance for the early disease diagnosis.

Bibliographic Details

Qingyi Han; Dingding Zhang; Rui Zhang; Jianpu Tang; Keying Xu; Mingzheng Shao; Yuyan Li; Peiyao Du; Ruizhong Zhang; Dayong Yang; Libing Zhang; Xiaoquan Lu

Elsevier BV

Materials Science; Physics and Astronomy; Engineering

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