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Protein kinase C differentially inhibits muscarinic receptor operated Ca 2+ release and entry in human salivary cells

Biochemical and Biophysical Research Communications, ISSN: 0006-291X, Vol: 152, Issue: 3, Page: 1062-1069
1988
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Article Description

We investigated the effects of phorbol myristate acetate on muscarinic receptor-induced Ca 2+ release from intracellular stores and extracellular entry in a human salivary duct cell line, HSG-PA. Phorbol myristate acetate (∼10 −7 M) blocked both Ca 2+ release and Ca 2+ entry induced by the muscarinic agonist carbachol. This blockade was the result of the activation of protein kinase C since 4α-phorbol 12,13-didecanoate, which lacks the ability to activate protein kinase C, did not inhibit Ca 2+ mobilization responses to carbachol. Importantly, at lower phorbol myristate acetate concentrations (∼10 −9 M), carbachol-induced Ca 2+ released was blocked, but carbachol-induced Ca 2+ entry was maintained. These results show that carbachol-induced Ca 2+ entry does not occur via an intracellular store and that protein kinase C plays a role in a feedback control mechanism for muscarinic-induced Ca 2+ mobilization at different levels.

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