Highly sensitive assay for PZ-peptidase activity by high-performance liquid chromatography
Journal of Chromatography A, ISSN: 0021-9673, Vol: 348, Issue: C, Page: 205-212
1985
- 7Citations
- 1Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations7
- Citation Indexes7
- CrossRef7
- Captures1
- Readers1
Article Description
A rapid and highly sensitive assay method for measuring PZ-peptidase activity in newborn rat brain is described. The method is based on monitoring the absorption at 320 nm of PZ-Pro-Leu enzymatically formed from the substrate, PZ- l -Pro- l -Leu-Gyl- l -Pro- d -Arg, after separation by high-performance liquid chromatography using a reversed-phase column. This method is sensitive enough to measure PZ-pro-Leu at concentrations as low as 5 pmol, and is able to make the column ready for the next injection within 10 min after the preceding injection. By using this method, PZ-peptidase activity was discovered in clonal osteoblastic cells derived from newborn mouse calvaria.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0021967301924549; http://dx.doi.org/10.1016/s0021-9673(01)92454-9; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0022349970&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/3910673; https://linkinghub.elsevier.com/retrieve/pii/S0021967301924549; http://linkinghub.elsevier.com/retrieve/pii/S0021967301924549; http://api.elsevier.com/content/article/PII:S0021967301924549?httpAccept=text/xml; http://api.elsevier.com/content/article/PII:S0021967301924549?httpAccept=text/plain; http://dx.doi.org/10.1016/s0021-9673%2801%2992454-9; https://dx.doi.org/10.1016/s0021-9673%2801%2992454-9
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