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Highly sensitive assay for PZ-peptidase activity by high-performance liquid chromatography

Journal of Chromatography A, ISSN: 0021-9673, Vol: 348, Issue: C, Page: 205-212
1985
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Article Description

A rapid and highly sensitive assay method for measuring PZ-peptidase activity in newborn rat brain is described. The method is based on monitoring the absorption at 320 nm of PZ-Pro-Leu enzymatically formed from the substrate, PZ- l -Pro- l -Leu-Gyl- l -Pro- d -Arg, after separation by high-performance liquid chromatography using a reversed-phase column. This method is sensitive enough to measure PZ-pro-Leu at concentrations as low as 5 pmol, and is able to make the column ready for the next injection within 10 min after the preceding injection. By using this method, PZ-peptidase activity was discovered in clonal osteoblastic cells derived from newborn mouse calvaria.

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