Blocking primers reduce co-amplification of plant DNA when studying bacterial endophyte communities.

Citation data:

Journal of microbiological methods, ISSN: 1872-8359, Vol: 117, Page: 1-3

Publication Year:
2015
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PMID:
26159909
DOI:
10.1016/j.mimet.2015.07.003
Author(s):
Arenz, Brett E, Schlatter, Dan C, Bradeen, James M, Kinkel, Linda L
Publisher(s):
Elsevier BV
Tags:
Immunology and Microbiology, Biochemistry, Genetics and Molecular Biology, Medicine
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article description
A blocking primer set based on the technique described by Vestheim and Jarman (2008) was developed to reduce amplification of non-target plant DNA when conducting metagenomic studies on bacterial endophyte communities. Bacterial amplification efficiency was increased 300-fold compared to standard PCR in an Illumina-based study of Sorghastrum nutans leaves.

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