Blocking primers reduce co-amplification of plant DNA when studying bacterial endophyte communities.

Citation data:

Journal of microbiological methods, ISSN: 1872-8359, Vol: 117, Page: 1-3

Publication Year:
2015
Usage 40
Abstract Views 23
Link-outs 17
Captures 64
Readers 64
Social Media 6
Tweets 4
Shares, Likes & Comments 2
Citations 5
Citation Indexes 5
PMID:
26159909
DOI:
10.1016/j.mimet.2015.07.003
Author(s):
Arenz, Brett E; Schlatter, Dan C; Bradeen, James M; Kinkel, Linda L
Publisher(s):
Elsevier BV
Tags:
Immunology and Microbiology; Biochemistry, Genetics and Molecular Biology; Medicine
Most Recent Tweet View All Tweets
article description
A blocking primer set based on the technique described by Vestheim and Jarman (2008) was developed to reduce amplification of non-target plant DNA when conducting metagenomic studies on bacterial endophyte communities. Bacterial amplification efficiency was increased 300-fold compared to standard PCR in an Illumina-based study of Sorghastrum nutans leaves.