Blocking primers reduce co-amplification of plant DNA when studying bacterial endophyte communities.
- Citation data:
Journal of microbiological methods, ISSN: 1872-8359, Vol: 117, Page: 1-3
- Publication Year:
- Immunology and Microbiology; Biochemistry, Genetics and Molecular Biology; Medicine
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A blocking primer set based on the technique described by Vestheim and Jarman (2008) was developed to reduce amplification of non-target plant DNA when conducting metagenomic studies on bacterial endophyte communities. Bacterial amplification efficiency was increased 300-fold compared to standard PCR in an Illumina-based study of Sorghastrum nutans leaves.