Spectral resolution of the primary electron acceptor A in photosystem i
Journal of Physical Chemistry B, ISSN: 1520-5207, Vol: 116, Issue: 10, Page: 3380-3386
2012
- 22Citations
- 25Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations22
- Citation Indexes22
- 22
- CrossRef19
- Captures25
- Readers25
- 25
Article Description
The reduced state of the primary electron acceptor of Photosystem I, A , was resolved spectroscopically in its lowest energy Q region for the first time without the addition of chemical reducing agents and without extensive data manipulation. To carry this out, we used the menB mutant of Synechocystis sp. PCC 6803 in which phylloquinone is replaced by plastoquinone-9 in the A sites of Photosystem I. The presence of plastoquinone-9 slows electron transfer from A to A, leading to a long-lived A state. This allows its spectral signature to be readily detected in a time-resolved optical pump-probe experiment. The maximum bleaching (A - A) was found to occur at 684 nm with a corresponding extinction coefficient of 43 mM cm. The data show evidence for an electrochromic shift of an accessory chlorophyll pigment, suggesting that the latter Q absorption band is centered around 682 nm. © 2012 American Chemical Society.
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