PlumX Metrics
Embed PlumX Metrics

Automated optogenetic feedback control for precise and robust regulation of gene expression and cell growth

Nature Communications, ISSN: 2041-1723, Vol: 7, Issue: 1, Page: 12546
2016
  • 171
    Citations
  • 0
    Usage
  • 354
    Captures
  • 2
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    171
  • Captures
    354
  • Mentions
    2
    • News Mentions
      2
      • News
        2

Most Recent News

Computer uses light to control ‘cyborg’ bacteria

A computer automatically controls the growth of new “cyborg” bacteria, report researchers. Two interfaces link machine and organism: the computer communicates with red and green light, which the biotechnologically modified bacteria can perceive. In the opposite direction, communication functions via an optical measurement of the growth rate of the bacteria culture, the results of which are fed int

Article Description

Dynamic control of gene expression can have far-reaching implications for biotechnological applications and biological discovery. Thanks to the advantages of light, optogenetics has emerged as an ideal technology for this task. Current state-of-the-art methods for optical expression control fail to combine precision with repeatability and cannot withstand changing operating culture conditions. Here, we present a novel fully automatic experimental platform for the robust and precise long-term optogenetic regulation of protein production in liquid Escherichia coli cultures. Using a computer-controlled light-responsive two-component system, we accurately track prescribed dynamic green fluorescent protein expression profiles through the application of feedback control, and show that the system adapts to global perturbations such as nutrient and temperature changes. We demonstrate the efficacy and potential utility of our approach by placing a key metabolic enzyme under optogenetic control, thus enabling dynamic regulation of the culture growth rate with potential applications in bacterial physiology studies and biotechnology.

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know