Automated optogenetic feedback control for precise and robust regulation of gene expression and cell growth
Nature Communications, ISSN: 2041-1723, Vol: 7, Issue: 1, Page: 12546
2016
- 171Citations
- 354Captures
- 2Mentions
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations171
- Citation Indexes170
- 170
- CrossRef162
- Patent Family Citations1
- Patent Families1
- Captures354
- Readers354
- 354
- Mentions2
- News Mentions2
- News2
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Article Description
Dynamic control of gene expression can have far-reaching implications for biotechnological applications and biological discovery. Thanks to the advantages of light, optogenetics has emerged as an ideal technology for this task. Current state-of-the-art methods for optical expression control fail to combine precision with repeatability and cannot withstand changing operating culture conditions. Here, we present a novel fully automatic experimental platform for the robust and precise long-term optogenetic regulation of protein production in liquid Escherichia coli cultures. Using a computer-controlled light-responsive two-component system, we accurately track prescribed dynamic green fluorescent protein expression profiles through the application of feedback control, and show that the system adapts to global perturbations such as nutrient and temperature changes. We demonstrate the efficacy and potential utility of our approach by placing a key metabolic enzyme under optogenetic control, thus enabling dynamic regulation of the culture growth rate with potential applications in bacterial physiology studies and biotechnology.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84984623464&origin=inward; http://dx.doi.org/10.1038/ncomms12546; http://www.ncbi.nlm.nih.gov/pubmed/27562138; https://www.nature.com/articles/ncomms12546; http://hdl.handle.net/20.500.11850/119985; http://dx.doi.org/10.3929/ethz-b-000119985; https://dx.doi.org/10.3929/ethz-b-000119985; https://www.research-collection.ethz.ch/handle/20.500.11850/119985; https://dx.doi.org/10.1038/ncomms12546; https://www.research-collection.ethz.ch/bitstream/20.500.11850/119985/2/ncomms12546.pdf; http://www.nature.com/doifinder/10.1038/ncomms12546; http://www.nature.com/articles/ncomms12546.pdf; http://www.nature.com/articles/ncomms12546
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