NeuBtracker-imaging neurobehavioral dynamics in freely behaving fish.

Citation data:

Nature methods, ISSN: 1548-7105, Vol: 14, Issue: 11, Page: 1079-1082

Publication Year:
2017
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PMID:
28967889
DOI:
10.1038/nmeth.4459
Author(s):
Symvoulidis, Panagiotis, Lauri, Antonella, Stefanoiu, Anca, Cappetta, Michele, Schneider, Steffen, Jia, Hongbo, Stelzl, Anja, Koch, Maximilian, Perez, Carlos Cruz, Myklatun, Ahne, Renninger, Sabine, Chmyrov, Andriy, Lasser, Tobias, Wurst, Wolfgang, Ntziachristos, Vasilis, Westmeyer, Gil G Show More Hide
Publisher(s):
Springer Nature
Tags:
Biochemistry, Genetics and Molecular Biology
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article description
A long-standing objective in neuroscience has been to image distributed neuronal activity in freely behaving animals. Here we introduce NeuBtracker, a tracking microscope for simultaneous imaging of neuronal activity and behavior of freely swimming fluorescent reporter fish. We showcase the value of NeuBtracker for screening neurostimulants with respect to their combined neuronal and behavioral effects and for determining spontaneous and stimulus-induced spatiotemporal patterns of neuronal activation during naturalistic behavior.

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