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Monitoring and imaging pH in biofilms utilizing a fluorescent polymeric nanosensor

Scientific Reports, ISSN: 2045-2322, Vol: 12, Issue: 1, Page: 9823
2022
  • 18
    Citations
  • 0
    Usage
  • 24
    Captures
  • 1
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    18
  • Captures
    24
  • Mentions
    1
    • News Mentions
      1
      • 1

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Wissenschaftliche Publikationen in Fachzeitschriften 2022

Wissenschaftliche Publikationen in Fachzeitschriften 2022 A Alarcan, J., G. de Sousa, E. S. Katsanou, A. Spyropoulou, P. Batakis, K. Machera, R. Rahmani, A. Lampen, Abdelhady

Article Description

Biofilms are ubiquitous in nature and in the man-made environment. Given their harmful effects on human health, an in-depth understanding of biofilms and the monitoring of their formation and growth are important. Particularly relevant for many metabolic processes and survival strategies of biofilms is their extracellular pH. However, most conventional techniques are not suited for minimally invasive pH measurements of living biofilms. Here, a fluorescent nanosensor is presented for ratiometric measurements of pH in biofilms in the range of pH 4.5–9.5 using confocal laser scanning microscopy. The nanosensor consists of biocompatible polystyrene nanoparticles loaded with pH-inert dye Nile Red and is surface functionalized with a pH-responsive fluorescein dye. Its performance was validated by fluorometrically monitoring the time-dependent changes in pH in E. coli biofilms after glucose inoculation at 37 °C and 4 °C. This revealed a temperature-dependent decrease in pH over a 4-h period caused by the acidifying glucose metabolism of E. coli. These studies demonstrate the applicability of this nanosensor to characterize the chemical microenvironment in biofilms with fluorescence methods.

Bibliographic Details

Kromer, Charlotte; Schwibbert, Karin; Gadicherla, Ashish K; Thiele, Dorothea; Nirmalananthan-Budau, Nithiya; Laux, Peter; Resch-Genger, Ute; Luch, Andreas; Tschiche, Harald R

Springer Science and Business Media LLC

Multidisciplinary

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