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Interaction of HapX with the CCAAT-binding complex - A novel mechanism of gene regulation by iron

EMBO Journal, ISSN: 0261-4189, Vol: 26, Issue: 13, Page: 3157-3168
2007
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Article Description

Iron homeostasis requires subtle control systems, as iron is both essential and toxic. In Aspergillus nidulans, iron represses iron acquisition via the GATA factor SreA, and induces iron-dependent pathways at the transcriptional level, by a so far unknown mechanism. Here, we demonstrate that iron-dependent pathways (e.g., heme biosynthesis) are repressed during iron-depleted conditions by physical interaction of HapX with the CCAAT-binding core complex (CBC). Proteome analysis identified putative HapX targets. Mutual transcriptional control between hapX and sreA and synthetic lethality resulting from deletion of both regulatory genes indicate a tight interplay of these control systems. Expression of genes encoding CBC subunits was not influenced by iron availability, and their deletion was deleterious during iron-depleted and iron-replete conditions. Expression of hapX was repressed by iron and its deletion was deleterious during iron-depleted conditions only. These data indicate that the CBC has a general role and that HapX function is confined to iron-depleted conditions. Remarkably, CBC-mediated regulation has an inverse impact on the expression of the same gene set in A. nidulans, compared with Saccharomyces cerevisae. © 2007 European Molecular Biology Organization | All Rights Reserved.

Bibliographic Details

Hortschansky, Peter; Eisendle, Martin; Al-Abdallah, Qusai; Schmidt, André D; Bergmann, Sebastian; Thön, Marcel; Kniemeyer, Olaf; Abt, Beate; Seeber, Birgit; Werner, Ernst R; Kato, Masashi; Brakhage, Axel A; Haas, Hubertus

Springer Science and Business Media LLC

Neuroscience; Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology

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