Evaluation of TCR Gene Editing Achieved by TALENs, CRISPR/Cas9, and megaTAL Nucleases.

Citation data:

Molecular therapy : the journal of the American Society of Gene Therapy, ISSN: 1525-0024, Vol: 24, Issue: 3, Page: 570-81

Publication Year:
2016
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PMID:
26502778
DOI:
10.1038/mt.2015.197
PMCID:
PMC4786913
Author(s):
Osborn, Mark J, Webber, Beau R, Knipping, Friederike, Lonetree, Cara-lin, Tennis, Nicole, DeFeo, Anthony P, McElroy, Amber N, Starker, Colby G, Lee, Catherine, Merkel, Sarah, Lund, Troy C, Kelly-Spratt, Karen S, Jensen, Michael C, Voytas, Daniel F, von Kalle, Christof, Schmidt, Manfred, Gabriel, Richard, Hippen, Keli L, Miller, Jeffrey S, Scharenberg, Andrew M, Tolar, Jakub, Blazar, Bruce R Show More Hide
Publisher(s):
Elsevier BV
Tags:
Biochemistry, Genetics and Molecular Biology, Pharmacology, Toxicology and Pharmaceutics
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article description
Present adoptive immunotherapy strategies are based on the re-targeting of autologous T-cells to recognize tumor antigens. As T-cell properties may vary significantly between patients, this approach can result in significant variability in cell potency that may affect therapeutic outcome. More consistent results could be achieved by generating allogeneic cells from healthy donors. An impediment to such an approach is the endogenous T-cell receptors present on T-cells, which have the potential to direct dangerous off-tumor antihost reactivity. To address these limitations, we assessed the ability of three different TCR-α-targeted nucleases to disrupt T-cell receptor expression in primary human T-cells. We optimized the conditions for the delivery of each reagent and assessed off-target cleavage. The megaTAL and CRISPR/Cas9 reagents exhibited the highest disruption efficiency combined with low levels of toxicity and off-target cleavage, and we used them for a translatable manufacturing process to produce safe cellular substrates for next-generation immunotherapies.

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