Oligoarginine peptides slow strand annealing and assist non-enzymatic RNA replication.

Citation data:

Nature chemistry, ISSN: 1755-4349, Vol: 8, Issue: 10, Page: 915-21

Publication Year:
2016
Usage 8
Abstract Views 5
Link-outs 3
Mentions 1
Blog Mentions 1
Social Media 19
Tweets 19
Citations 24
Citation Indexes 24
PMID:
27657866
DOI:
10.1038/nchem.2551
PMCID:
PMC5061144
Author(s):
Jia, Tony Z, Fahrenbach, Albert C, Kamat, Neha P, Adamala, Katarzyna P, Szostak, Jack W
Publisher(s):
Springer Nature
Tags:
Chemistry, Chemical Engineering
Most Recent Tweet View All Tweets
Most Recent Blog Mention
article description
The non-enzymatic replication of RNA is thought to have been a critical process required for the origin of life. One unsolved difficulty with non-enzymatic RNA replication is that template-directed copying of RNA results in a double-stranded product. After strand separation, rapid strand reannealing outcompetes slow non-enzymatic template copying, which renders multiple rounds of RNA replication impossible. Here we show that oligoarginine peptides slow the annealing of complementary oligoribonucleotides by up to several thousand-fold; however, short primers and activated monomers can still bind to template strands, and template-directed primer extension can still occur, all within a phase-separated condensed state, or coacervate. Furthermore, we show that within this phase, partial template copying occurs even in the presence of full-length complementary strands. This method to enable further rounds of replication suggests one mechanism by which short non-coded peptides could have enhanced early cellular fitness, and potentially explains how longer coded peptides, that is, proteins, came to prominence in modern biology.

This article has 0 Wikipedia mention.