Engineering genetic circuit interactions within and between synthetic minimal cells.

Citation data:

Nature chemistry, ISSN: 1755-4349, Vol: 9, Issue: 5, Page: 431-439

Publication Year:
Usage 15
Clicks 9
Abstract Views 4
Link-outs 2
Captures 210
Readers 210
Mentions 2
News Mentions 2
Social Media 61
Tweets 60
Shares, Likes & Comments 1
Citations 15
Citation Indexes 15
Adamala, Katarzyna P; Martin-Alarcon, Daniel A; Guthrie-Honea, Katriona R; Boyden, Edward S
Springer Nature
Chemistry; Chemical Engineering
Most Recent Tweet View All Tweets
Most Recent News Mention
article description
Genetic circuits and reaction cascades are of great importance for synthetic biology, biochemistry and bioengineering. An open question is how to maximize the modularity of their design to enable the integration of different reaction networks and to optimize their scalability and flexibility. One option is encapsulation within liposomes, which enables chemical reactions to proceed in well-isolated environments. Here we adapt liposome encapsulation to enable the modular, controlled compartmentalization of genetic circuits and cascades. We demonstrate that it is possible to engineer genetic circuit-containing synthetic minimal cells (synells) to contain multiple-part genetic cascades, and that these cascades can be controlled by external signals as well as inter-liposomal communication without crosstalk. We also show that liposomes that contain different cascades can be fused in a controlled way so that the products of incompatible reactions can be brought together. Synells thus enable a more modular creation of synthetic biology cascades, an essential step towards their ultimate programmability.