Cell-cell communication mimicry with poly(ethylene glycol) hydrogels for enhancing β-cell function
Proceedings of the National Academy of Sciences of the United States of America, ISSN: 0027-8424, Vol: 108, Issue: 16, Page: 6380-6385
2011
- 148Citations
- 225Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations148
- Citation Indexes147
- 147
- CrossRef137
- Patent Family Citations1
- Patent Families1
- Captures225
- Readers225
- 204
- 21
Article Description
A biomimetic hydrogel platform was designed to signal encapsulated cells using immobilized cell-cell communication cues, with a focus on enhancing the survival and function of encapsulated pancreatic β-cells to treat type 1 diabetes. When MIN6 cells, a pancreatic β-cell line, were encapsulated in poly(ethylene glycol) (PEG) hydrogels, their survival and glucose responsiveness to insulin were highly dependent on the cell-packing density. A minimum packing density of 107 cells/mL was necessary to maintain the survival of encapsulated β-cells without the addition of material functionalities (e.g., cell adhesion ligands). While single cell suspensions can improve diffusion-limited mass transfer, direct cell-cell interactions are limited. Thus, thiolated EphA5-Fc receptor and ephrinA5-Fc ligand were conjugated into PEG hydrogels via a thiol-acrylate photopolymerization to render an otherwise inert PEG hydrogel bioactive. The biomimetic hydrogels presented here can provide crucial cell-cell communication signals for dispersed β-cells and improve their survival and proliferation. Together with the cell-adhesive peptide RGDS, the immobilized fusion proteins (EphA5-Fc and ephrinA5-Fc) synergistically increased the survival of both MIN6 β-cells and dissociated islet cells, both at a very low cell-packing density (<2 × 10 cells/mL). This unique gel platform demonstrates new strategies for tailoring biomimetic environments to enhance the encapsulation of cells that require cell-cell contact to survive and function.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=79955595400&origin=inward; http://dx.doi.org/10.1073/pnas.1014026108; http://www.ncbi.nlm.nih.gov/pubmed/21464290; https://pnas.org/doi/full/10.1073/pnas.1014026108; https://dx.doi.org/10.1073/pnas.1014026108; https://www.pnas.org/content/108/16/6380
Proceedings of the National Academy of Sciences
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