Reconstitution of Yeast Nucleotide Excision Repair with Purified Rad Proteins, Replication Protein A, and Transcription Factor TFIIH ∗
Journal of Biological Chemistry, ISSN: 0021-9258, Vol: 270, Issue: 22, Page: 12973-12976
1995
- 211Citations
- 52Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations211
- Citation Indexes209
- 209
- CrossRef168
- Policy Citations2
- Policy Citation2
- Captures52
- Readers52
- 52
Article Description
Nucleotide excision repair (NER) functions to remove DNA damage caused by ultraviolet light and by other agents that distort the DNA helix. The NER machinery has been conserved in structure and function from yeast to humans, and in humans, defective NER is the underlying cause of the cancer-prone disease xeroderma pigmentosum. Here, we reconstitute the incision reaction of NER in Saccharomyces cerevisiae using purified protein factors. The Rad14 protein, the Rad4-Rad23 complex, the Rad2 nuclease, the Rad1-Rad10 nuclease, replication protein A, and the RNA polymerase II transcription factor TFIIH were purified to near homogeneity from yeast. We show that these protein factors are both necessary and sufficient for dual incision of DNA damaged by either ultraviolet light or N -acetoxy-2-aminoacetylfluorene. Incision in the reconstituted system requires ATP, which cannot be substituted by adenosine 5′- O -(3-thiotriphosphate), suggesting that the hydrolysis of ATP is indispensable for the incision reaction. The excision DNA fragments formed as a result of dual incision are in the 24-27-nucleotide range.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0021925818922325; http://dx.doi.org/10.1074/jbc.270.22.12973; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0029019788&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/7768886; https://linkinghub.elsevier.com/retrieve/pii/S0021925818922325; https://dx.doi.org/10.1074/jbc.270.22.12973
Elsevier BV
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