Tyrosine phosphorylation of Eps15 is required for ligand-regulated, but not constitutive, endocytosis
Journal of Cell Biology, ISSN: 0021-9525, Vol: 150, Issue: 4, Page: 905-911
2000
- 119Citations
- 58Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations119
- Citation Indexes119
- 119
- CrossRef117
- Captures58
- Readers58
- 58
Article Description
Membrane receptors are internalized either constitutively or upon ligand engagement. Whereas there is evidence for differential regulation of the two processes, little is known about the molecular machinery involved. Previous studies have shown that an unidentified kinase substrate is required for endocytosis of the epidermal growth factor receptor (EGFR), the prototypical ligand-inducible receptor, but not of the transferrin receptor (TfR), the prototypical constitutively internalized receptor. Eps15, an endocytic protein that is tyrosine phosphorylated by EGFR, is a candidate for such a function. Here, we show that tyrosine phosphorylation of Eps15 is necessary for internalization of the EGFR, but not of the TfR. We mapped Tyr 850 as the major in vivo tyrosine phosphorylation site of Eps15. A phosphorylation-negative mutant of Eps15 acted as a dominant negative on the internalization of the EGFR, but not of the TfR. A phosphopeptide, corresponding to the phosphorylated sequence of Eps15, inhibited EGFR endocytosis, suggesting that phosphotyrosine in Eps15 serves as a docking site for a phosphotyrosine binding protein. Thus, tyrosine phosphorylation of Eps15 represents the first molecular determinant, other than those contained in the receptors themselves, which is involved in the differential regulation of constitutive vs. regulated endocytosis.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0034698787&origin=inward; http://dx.doi.org/10.1083/jcb.150.4.905; http://www.ncbi.nlm.nih.gov/pubmed/10953014; https://rupress.org/jcb/article/150/4/905/45483/Tyrosine-Phosphorylation-of-Eps15-Is-Required-for; https://dx.doi.org/10.1083/jcb.150.4.905
Rockefeller University Press
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